› 2ie2

X-ray diffraction
1.7Å resolution

The 1.7 A crystal structure of Dronpa: a photoswitchable green fluorescent protein

Released:
DOI: 10.2210/pdb2ie2/pdb
PDB entry 2ie2 coloured by chain, front view.
PDB entry 2ie2 coloured by chain, side view.
PDB entry 2ie2 coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
The 1.7 A crystal structure of Dronpa: a photoswitchable green fluorescent protein.
Wilmann PG, Turcic K, Battad JM, Wilce MC, Devenish RJ, Prescott M, Rossjohn J
J Mol Biol 364 213-24 (2006)
PMID: 17010376

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 222 amino acids
Theoretical weight: 25.5 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 6 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21212
Unit cell:
a: 103.14Å b: 175.499Å c: 67.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.198 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
7 more

3 mentions without citation

A bright and photostable photoconvertible fluorescent protein.
McKinney et al. (2009)
2 more