› 2ieh

X-ray diffraction
2.7Å resolution

Crystal structure of human kinesin Eg5 in complex with (R)-mon97, a new monastrol-based inhibitor that binds as (R)-enantiomer

Released:
DOI: 10.2210/pdb2ieh/pdb
PDB entry 2ieh coloured by chain, front view.
PDB entry 2ieh coloured by chain, side view.
PDB entry 2ieh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human Eg5 in complex with a new monastrol-based inhibitor bound in the R configuration.
Garcia-Saez I, DeBonis S, Lopez R, Trucco F, Rousseau B, Thuéry P, Kozielski F
J Biol Chem 282 9740-9747 (2007)
PMID: 17251189

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin-like protein KIF11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 40.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52732 (Residues: 2-368; Coverage: 35%)
Gene names: EG5, KIF11, KNSL1, TRIP5
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

6 bound ligands:
Ligand MG 2 x MG
Ligand K 1 x K
Ligand CL 1 x CL
Ligand ADP 2 x ADP
Ligand MOY 2 x MOY
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 69.37Å b: 79.9Å c: 159.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.281
Expression system: Escherichia coli

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Citations

1 review citation

Highlights on U.S. FDA-approved fluorinated drugs over the past five years (2018-2022).
Ali et al. (2023)
26 other articles

4 mentions without citation

Recent findings and future directions for interpolar mitotic kinesin inhibitors in cancer therapy.
Myers et al. (2016)
3 more