› 2if6

X-ray diffraction
1.8Å resolution

Crystal structure of metalloprotein yiiX from Escherichia coli O157:H7, DUF1105

Released:
DOI: 10.2210/pdb2if6/pdb
PDB entry 2if6 coloured by chain, front view.
PDB entry 2if6 coloured by chain, side view.
PDB entry 2if6 coloured by chain, top view.
Source organism: Escherichia coli O157:H7
Entry authors: Bonanno JB, Gilmore J, Bain KT, Powell A, Ozyurt S, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipid binding hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 186 amino acids
Theoretical weight: 21.42 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8X778 (Residues: 19-202; Coverage: 91%)
Gene names: ECs_4866, yiiX
Sequence domains: Permuted papain-like amidase enzyme, YaeF/YiiX, C92 family
Structure domains: endopeptidase domain like (from Nostoc punctiforme)

Ligands and Environments

1 bound ligand:
Ligand UNX 2 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 32.345Å b: 152.594Å c: 48.74Å
α: 90° β: 92.67° γ: 90°
R-values:
R R work R free
0.181 0.179 0.231
Expression system: Escherichia coli BL21(DE3)

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