› 2ifu

X-ray diffraction
2.6Å resolution

Crystal Structure of a Gamma-SNAP from Danio rerio

Released:
DOI: 10.2210/pdb2ifu/pdb
PDB entry 2ifu coloured by chain, front view.
PDB entry 2ifu coloured by chain, side view.
PDB entry 2ifu coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structure and dynamics of gamma-SNAP: insight into flexibility of proteins from the SNAP family.
Bitto E, Bingman CA, Kondrashov DA, McCoy JG, Bannen RM, Wesenberg GE, Phillips GN
Proteins 70 93-104 (2008)
PMID: 17634982

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176895 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-soluble NSF attachment protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 307 amino acids
Theoretical weight: 34.59 KDa
Source organism: Danio rerio
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5BJK3 (Residues: 2-305; Coverage: 100%)
Gene names: napga, napgl, zgc:110177
Sequence domains: Soluble NSF attachment protein, SNAP
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D, APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 83.378Å b: 90.813Å c: 264.173Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.261
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).
Zhao et al. (2012)
1 more

3 mentions without citation

COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis.
Stuttmann et al. (2009)
2 more