› 2igi

X-ray diffraction
1.7Å resolution

Crystal Structure of E. coli Oligoribonuclease

Released:
DOI: 10.2210/pdb2igi/pdb
PDB entry 2igi coloured by chain, front view.
PDB entry 2igi coloured by chain, side view.
PDB entry 2igi coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Zuo Y, Malhotra A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141551 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oligoribonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.71 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A784 (Residues: 2-181; Coverage: 99%)
Gene names: JW5740, b4162, o204a, orn, yjeR
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand CD 13 x CD
Ligand ACY 8 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P65
Unit cell:
a: 91.55Å b: 91.55Å c: 111.18Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.177 0.177 0.2
Expression system: Escherichia coli BL21(DE3)

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