› 2ih9

X-ray diffraction
2Å resolution

A high-dose crystal structure of a recombinant Melanocarbus albomyces laccase

Released:
DOI: 10.2210/pdb2ih9/pdb
PDB entry 2ih9 coloured by chain, front view.
PDB entry 2ih9 coloured by chain, side view.
PDB entry 2ih9 coloured by chain, top view.
Source organism: Melanocarpus albomyces
Primary publication:
A crystallographic and spectroscopic study on the effect of X-ray radiation on the crystal structure of Melanocarpus albomyces laccase.
Hakulinen N, Kruus K, Koivula A, Rouvinen J
Biochem Biophys Res Commun 350 929-34 (2006)
PMID: 17045575

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Laccase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 559 amino acids
Theoretical weight: 61.85 KDa
Source organism: Melanocarpus albomyces
Expression system: Trichoderma reesei
UniProt:
  • Canonical: Q70KY3 (Residues: 51-609; Coverage: 93%)
Gene name: LAC1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
4 bound ligands:
Ligand NAG 10 x NAG
Ligand CU 8 x CU
Ligand CL 2 x CL
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2
Unit cell:
a: 174.49Å b: 62.33Å c: 125.1Å
α: 90° β: 96.25° γ: 90°
R-values:
R R work R free
0.263 0.263 0.299
Expression system: Trichoderma reesei

Quick links

Citations

10 review citations

Copper active sites in biology.
Solomon et al. (2014)
9 more