› 2iho

X-ray diffraction
2.41Å resolution

Crystal structure of MOA, a lectin from the mushroom Marasmius oreades in complex with the trisaccharide Gal(1,3)Gal(1,4)GlcNAc

Released:
DOI: 10.2210/pdb2iho/pdb
PDB entry 2iho coloured by chain, front view.
PDB entry 2iho coloured by chain, side view.
PDB entry 2iho coloured by chain, top view.
Source organism: Marasmius oreades
Primary publication:
Crystal structure of the Marasmius oreades mushroom lectin in complex with a xenotransplantation epitope.
Grahn E, Askarieh G, Holmner A, Tateno H, Winter HC, Goldstein IJ, Krengel U
J Mol Biol 369 710-21 (2007)
PMID: 17442345

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186825 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Agglutinin Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 32.33 KDa
Source organism: Marasmius oreades
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8X123 (Residues: 1-293; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 104.64Å b: 104.64Å c: 112.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.19 0.211
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Bioactive proteins from mushrooms.
Xu et al. (2011)
5 more

13 mentions without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)
12 more