› 2iid

X-ray diffraction
1.8Å resolution

Structure of L-amino acid oxidase from Calloselasma rhodostoma in complex with L-phenylalanine

Released:
DOI: 10.2210/pdb2iid/pdb
PDB entry 2iid coloured by chain, front view.
PDB entry 2iid coloured by chain, side view.
PDB entry 2iid coloured by chain, top view.
Source organism: Calloselasma rhodostoma
Primary publication:
Crystal structure of LAAO from Calloselasma rhodostoma with an L-phenylalanine substrate: insights into structure and mechanism.
Moustafa IM, Foster S, Lyubimov AY, Vrielink A
J Mol Biol 364 991-1002 (2006)
PMID: 17046020

Function and Biology Details

Reaction catalysed: 
An L-amino acid + H(2)O + O(2) = a 2-oxo carboxylate + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160512 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
L-amino-acid oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 498 amino acids
Theoretical weight: 56.3 KDa
Source organism: Calloselasma rhodostoma
UniProt:
  • Canonical: P81382 (Residues: 19-516; Coverage: 100%)
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand PHE 4 x PHE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 78.76Å b: 154.003Å c: 103.183Å
α: 90° β: 109.52° γ: 90°
R-values:
R R work R free
0.174 0.172 0.21

Quick links

Citations

14 review citations

Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis.
Kang et al. (2011)
13 more

14 mentions without citation

Snake venom L-amino acid oxidases: trends in pharmacology and biochemistry.
Izidoro et al. (2014)
13 more