› 2ikf

X-ray diffraction
2Å resolution

Terminal uridylyl transferase 4 from Trypanosoma brucei with bound UTP

Released:
DOI: 10.2210/pdb2ikf/pdb
PDB entry 2ikf coloured by chain, front view.
PDB entry 2ikf coloured by chain, side view.
PDB entry 2ikf coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
UTP-bound and Apo structures of a minimal RNA uridylyltransferase.
Stagno J, Aphasizheva I, Rosengarth A, Luecke H, Aphasizhev R
J Mol Biol 366 882-99 (2007)
PMID: 17189640

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 353 amino acids
Theoretical weight: 39.88 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q381M1 (Residues: 1-333; Coverage: 100%)
Gene names: TUT4, Tb11.01.7300
Sequence domains: Cid1 family poly A polymerase

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand UTP 1 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 78.773Å b: 41.525Å c: 103.792Å
α: 90° β: 94.94° γ: 90°
R-values:
R R work R free
0.228 0.228 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
10 more

12 mentions without citation

Determinants of substrate specificity in RNA-dependent nucleotidyl transferases.
Martin et al. (2008)
11 more