› 2iks

X-ray diffraction
1.85Å resolution

Crystal structure of N-terminal truncated DNA-binding transcriptional dual regulator from Escherichia coli K12

Released:
DOI: 10.2210/pdb2iks/pdb
PDB entry 2iks coloured by chain, front view.
PDB entry 2iks coloured by chain, side view.
PDB entry 2iks coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Chang C, Evdokimova E, Kagan O, Savchenko A, Edwards AM, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142289 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catabolite repressor/activator Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 33.73 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACP1 (Residues: 44-334; Coverage: 87%)
Gene names: JW0078, b0080, cra, fruC, fruR, shl
Sequence domains: Periplasmic binding proteins and sugar binding domain of LacI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 152.931Å b: 41.829Å c: 110.674Å
α: 90° β: 126.18° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression system: Escherichia coli

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