› 2in3

X-ray diffraction
1.85Å resolution

Crystal structure of a putative protein disulfide isomerase from Nitrosomonas europaea

Released:
DOI: 10.2210/pdb2in3/pdb
PDB entry 2in3 coloured by chain, front view.
PDB entry 2in3 coloured by chain, side view.
PDB entry 2in3 coloured by chain, top view.
Source organism: Nitrosomonas europaea
Entry authors: Cuff ME, Skarina T, Onopriyenko O, Edwards A, Savchenko A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DSBA-like thioredoxin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.44 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82S11 (Residues: 1-212; Coverage: 100%)
Gene name: NE2553
Sequence domains: DSBA-like thioredoxin domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 1 x PO4
Ligand EDO 4 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 48.844Å b: 59.642Å c: 84.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.227
Expression system: Escherichia coli BL21(DE3)

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