› 2inw

X-ray diffraction
1.5Å resolution

Crystal structure of Q83JN9 from Shigella flexneri at high resolution. Northeast Structural Genomics Consortium target SfR137.

Released:
DOI: 10.2210/pdb2inw/pdb
PDB entry 2inw coloured by chain, front view.
PDB entry 2inw coloured by chain, side view.
PDB entry 2inw coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems.
Arbing MA, Handelman SK, Kuzin AP, Verdon G, Wang C, Su M, Rothenbacher FP, Abashidze M, Liu M, Hurley JM, Xiao R, Acton T, Inouye M, Montelione GT, Woychik NA, Hunt JF
Structure 18 996-1010 (2010)
PMID: 20696400

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101581 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Structural protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.96 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2VX33 (Residues: 1-125; Coverage: 100%)
Gene names: SF2998, yeeU
Sequence domains: CbeA_antitoxin, type IV, cytoskeleton bundling-enhancing factor A
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 31.499Å b: 73.967Å c: 110.698Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.25
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Toxin-antitoxin systems in bacteria and archaea.
Yamaguchi et al. (2011)
20 more

2 mentions without citation

Structure, Biology, and Therapeutic Application of Toxin-Antitoxin Systems in Pathogenic Bacteria.
Lee et al. (2016)
1 more