Function and Biology Details
Reactions catalysed:
Glutathionylspermidine + H(2)O = glutathione + spermidine
Glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional glutathionylspermidine synthetase/amidase
Molecule details ›
Chains: A, B
Length: 619 amino acids
Theoretical weight: 70.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains: endopeptidase domain like (from Nostoc punctiforme)
Length: 619 amino acids
Theoretical weight: 70.61 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
- Canonical:
P0AES0 (Residues: 1-619; Coverage: 100%)
Sequence domains:
Structure domains: endopeptidase domain like (from Nostoc punctiforme)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SPRING-8 BEAMLINE BL12B2
Spacegroup:
C2
Expression system: Escherichia coli
