› 2iov

X-ray diffraction
1.8Å resolution

Bright-state structure of the reversibly switchable fluorescent protein Dronpa

Released:
DOI: 10.2210/pdb2iov/pdb
PDB entry 2iov coloured by chain, front view.
PDB entry 2iov coloured by chain, side view.
PDB entry 2iov coloured by chain, top view.
Source organism: Echinophyllia sp. SC22
Primary publication:
1.8 A bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants.
Stiel AC, Trowitzsch S, Weber G, Andresen M, Eggeling C, Hell SW, Jakobs S, Wahl MC
Biochem J 402 35-42 (2007)
PMID: 17117927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178339 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 255 amino acids
Theoretical weight: 29.25 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 4 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 75.292Å b: 109.627Å c: 275.232Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.216
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Super-resolution fluorescence microscopy.
Huang et al. (2009)
34 more

10 mentions without citation

Following Optogenetic Dimerizers and Quantitative Prospects.
Niu et al. (2016)
9 more