› 2ip1

X-ray diffraction
1.8Å resolution

Crystal Structure Analysis of S. cerevisiae Tryptophanyl tRNA Synthetase

Released:
DOI: 10.2210/pdb2ip1/pdb
PDB entry 2ip1 coloured by chain, front view.
PDB entry 2ip1 coloured by chain, side view.
PDB entry 2ip1 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.
Malkowski MG, Quartley E, Friedman AE, Babulski J, Kon Y, Wolfley J, Said M, Luft JR, Phizicky EM, DeTitta GT, Grayhack EJ
Proc Natl Acad Sci U S A 104 6678-83 (2007)
PMID: 17426150

Function and Biology Details

Reaction catalysed: 
ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171294 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan--tRNA ligase, cytoplasmic Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 49.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12109 (Residues: 1-432; Coverage: 100%)
Gene names: HRE432, WRS1, YOL097C
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PG4 4 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P43212
Unit cell:
a: 56.35Å b: 56.35Å c: 313.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.214
Expression system: Saccharomyces cerevisiae

Quick links

Citations

9 review citations

Selenium and anticarcinogenesis: underlying mechanisms.
Jackson et al. (2008)
8 more

3 mentions without citation

Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing.
Malkowski et al. (2007)
2 more