› 2ipi

X-ray diffraction
1.65Å resolution

Crystal Structure of Aclacinomycin Oxidoreductase

Released:
DOI: 10.2210/pdb2ipi/pdb
PDB entry 2ipi coloured by chain, front view.
PDB entry 2ipi coloured by chain, side view.
PDB entry 2ipi coloured by chain, top view.
Source organism: Streptomyces galilaeus
Primary publication:
Structure determination by multiwavelength anomalous diffraction of aclacinomycin oxidoreductase: indications of multidomain pseudomerohedral twinning.
Sultana A, Alexeev I, Kursula I, Mäntsälä P, Niemi J, Schneider G
Acta Crystallogr D Biol Crystallogr 63 149-59 (2007)
PMID: 17242508

Function and Biology Details

Reactions catalysed: 
Aclacinomycin N + O(2) = aclacinomycin A + H(2)O(2)
Aclacinomycin A + O(2) = aclacinomycin Y + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171071 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aclacinomycin-N/aclacinomycin-A oxidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 521 amino acids
Theoretical weight: 57.22 KDa
Source organism: Streptomyces galilaeus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0PCD7 (Residues: 44-545; Coverage: 100%)
Gene name: aknOx
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:
Ligand AKY 1 x AKY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 68.5Å b: 266.2Å c: 68.7Å
α: 90° β: 119° γ: 90°
R-values:
R R work R free
0.185 not available 0.242
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Rethinking Biosynthesis of Aclacinomycin A.
Xu et al. (2023)
5 other articles

3 mentions without citation

Aclacinomycin oxidoreductase (AknOx) from the biosynthetic pathway of the antibiotic aclacinomycin is an unusual flavoenzyme with a dual active site.
Alexeev et al. (2007)
2 more