› 2is1

X-ray diffraction
2.9Å resolution

Crystal structure of UvrD-DNA-SO4 complex

Released:
DOI: 10.2210/pdb2is1/pdb
PDB entry 2is1 coloured by chain, front view.
PDB entry 2is1 coloured by chain, side view.
PDB entry 2is1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.
Lee JY, Yang W
Cell 127 1349-60 (2006)
PMID: 17190599

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115406 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA helicase II Chains: A, B
Molecule details ›
Chains: A, B
Length: 680 amino acids
Theoretical weight: 77.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03018 (Residues: 1-680; Coverage: 94%)
Gene names: JW3786, b3813, mutU, pdeB, rad, recL, uvrD
Sequence domains:
Structure domains:
5'-D(*GP*CP*AP*GP*TP*GP*CP*TP*CP*GP*TP*TP*TP*TP*TP*TP*T)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 17 nucleotides
Theoretical weight: 5.19 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*GP*AP*GP*CP*AP*CP*TP*GP*C)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand HG 5 x HG
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 152.451Å b: 94.49Å c: 136.443Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.295
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

53 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
52 more

14 mentions without citation

Structural frameworks for considering microbial protein- and nucleic acid-dependent motor ATPases.
Thomsen et al. (2008)
13 more