› 2is3

X-ray diffraction
3.1Å resolution

Crystal Structure of Escherichia coli RNase T

Released:
DOI: 10.2210/pdb2is3/pdb
PDB entry 2is3 coloured by chain, front view.
PDB entry 2is3 coloured by chain, side view.
PDB entry 2is3 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of RNase T, an exoribonuclease involved in tRNA maturation and end turnover.
Zuo Y, Zheng H, Wang Y, Chruszcz M, Cymborowski M, Skarina T, Savchenko A, Malhotra A, Minor W
Structure 15 417-28 (2007)
PMID: 17437714

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo 32-mer
homo hexadecamer
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-151607 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease T Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 215 amino acids
Theoretical weight: 23.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30014 (Residues: 1-215; Coverage: 100%)
Gene names: JW1644, b1652, rnt
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

1 bound ligand:
Ligand SO4 13 x SO4
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: I422
Unit cell:
a: 213.14Å b: 213.14Å c: 149.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The critical role of RNA processing and degradation in the control of gene expression.
Arraiano et al. (2010)
1 more

4 mentions without citation

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
3 more