› 2is6

X-ray diffraction
2.2Å resolution

Crystal structure of UvrD-DNA-ADPMgF3 ternary complex

Released:
DOI: 10.2210/pdb2is6/pdb
PDB entry 2is6 coloured by chain, front view.
PDB entry 2is6 coloured by chain, side view.
PDB entry 2is6 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
UvrD helicase unwinds DNA one base pair at a time by a two-part power stroke.
Lee JY, Yang W
Cell 127 1349-60 (2006)
PMID: 17190599

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115409 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA helicase II Chains: A, B
Molecule details ›
Chains: A, B
Length: 680 amino acids
Theoretical weight: 77.8 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03018 (Residues: 1-680; Coverage: 94%)
Gene names: JW3786, b3813, mutU, pdeB, rad, recL, uvrD
Sequence domains:
Structure domains:
5'-D(*CP*GP*AP*GP*CP*AP*CP*TP*GP*CP*AP*GP*TP*GP*CP*TP*CP*GP*TP*TP*GP*TP*TP*AP*T)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 25 nucleotides
Theoretical weight: 7.68 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand MGF 2 x MGF
Ligand ADP 2 x ADP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 102.336Å b: 96.609Å c: 110.93Å
α: 90° β: 94.05° γ: 90°
R-values:
R R work R free
0.21 0.21 0.24
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

53 review citations

SF1 and SF2 helicases: family matters.
Fairman-Williams et al. (2010)
52 more

8 mentions without citation

XPB and XPD helicases in TFIIH orchestrate DNA duplex opening and damage verification to coordinate repair with transcription and cell cycle via CAK kinase.
Fuss et al. (2011)
7 more