› 2isn

X-ray diffraction
1.9Å resolution

Crystal structure of a phosphatase from a pathogenic strain Toxoplasma gondii

Released:
DOI: 10.2210/pdb2isn/pdb
PDB entry 2isn coloured by chain, front view.
PDB entry 2isn coloured by chain, side view.
PDB entry 2isn coloured by chain, top view.
Source organism: Toxoplasma gondii
Primary publication:
Structural genomics of protein phosphatases.
Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK
J Struct Funct Genomics 8 121-40 (2007)
PMID: 18058037

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NYSGXRC-8828z, phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 40.23 KDa
Source organism: Toxoplasma gondii
Expression system: Escherichia coli
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand PR 2 x PR
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C, NSLS BEAMLINE X25
Spacegroup: P1
Unit cell:
a: 52.986Å b: 62.447Å c: 63.738Å
α: 65.53° β: 70.21° γ: 77.64°
R-values:
R R work R free
0.241 0.241 0.279
Expression system: Escherichia coli

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Citations

24 review citations

Aiding and abetting roles of NOX oxidases in cellular transformation.
Block et al. (2012)
23 more

3 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
2 more