› 2ist

X-ray diffraction
1.86Å resolution

crystal structure of RluD from E. coli

Released:
DOI: 10.2210/pdb2ist/pdb
PDB entry 2ist coloured by chain, front view.
PDB entry 2ist coloured by chain, side view.
PDB entry 2ist coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Foster PG, Finer-Moore JS, Stroud RM

Function and Biology Details

Reaction catalysed: 
23S rRNA uridine(1911)/uridine(1915)/uridine(1917) = 23S rRNA pseudouridine(1911)/pseudouridine(1915)/pseudouridine(1917)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal large subunit pseudouridine synthase D Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 37.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P33643 (Residues: 2-326; Coverage: 100%)
Gene names: JW2576, b2594, rluD, sfhB, yfiI
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand BCT 2 x BCT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 67.32Å b: 75.14Å c: 85.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.199 0.241
Expression system: Escherichia coli

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