› 2isy

X-ray diffraction
1.96Å resolution

Crystal structure of the nickel-activated two-domain iron-dependent regulator (IdeR)

Released:
DOI: 10.2210/pdb2isy/pdb
PDB entry 2isy coloured by chain, front view.
PDB entry 2isy coloured by chain, side view.
PDB entry 2isy coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structures, metal activation, and DNA-binding properties of two-domain IdeR from Mycobacterium tuberculosis.
Wisedchaisri G, Chou CJ, Wu M, Roach C, Rice AE, Holmes RK, Beeson C, Hol WG
Biochemistry 46 436-47 (2007)
PMID: 17209554

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-dependent repressor IdeR Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 amino acids
Theoretical weight: 17.87 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMH1 (Residues: 1-140; Coverage: 61%)
Gene names: MTCY05A6.32, Rv2711, dtxR, ideR
Sequence domains:
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

2 bound ligands:
Ligand NI 4 x NI
Ligand PO4 2 x PO4
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 41.954Å b: 44.743Å c: 49.641Å
α: 110.18° β: 96.57° γ: 108.29°
R-values:
R R work R free
0.178 0.176 0.211
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Metal sensor proteins: nature's metalloregulated allosteric switches.
Giedroc et al. (2007)
5 more

6 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
5 more