2isz

X-ray diffraction
2.4Å resolution

Crystal structure of a two-domain IdeR-DNA complex crystal form I

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-115412 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Iron-dependent repressor IdeR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 157 amino acids
Theoretical weight: 17.86 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMH1 (Residues: 1-140; Coverage: 61%)
Gene names: MTCY05A6.32, Rv2711, dtxR, ideR
Sequence domains:
Structure domains:
mbtA/mbtB operator strand 1 Chain: E
Molecule details ›
Chain: E
Length: 33 nucleotides
Theoretical weight: 10.11 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided
mbtA/mbtB operator strand 2 Chain: F
Molecule details ›
Chain: F
Length: 33 nucleotides
Theoretical weight: 10.19 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 53.832Å b: 69.739Å c: 76.461Å
α: 106.57° β: 104.85° γ: 99.66°
R-values:
R R work R free
0.201 0.199 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided