› 2iw3

X-ray diffraction
2.4Å resolution

Elongation Factor 3 in complex with ADP

Released:
DOI: 10.2210/pdb2iw3/pdb
PDB entry 2iw3 coloured by chain, front view.
PDB entry 2iw3 coloured by chain, side view.
PDB entry 2iw3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of eEF3 and the mechanism of transfer RNA release from the E-site.
Andersen CB, Becker T, Blau M, Anand M, Halic M, Balar B, Mielke T, Boesen T, Pedersen JS, Spahn CM, Kinzy TG, Andersen GR, Beckmann R
Nature 443 663-8 (2006)
PMID: 16929303

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor 3A Chains: A, B
Molecule details ›
Chains: A, B
Length: 986 amino acids
Theoretical weight: 110.7 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P16521 (Residues: 2-981; Coverage: 94%)
Gene names: EFC1, L9672.5, TEF3, YEF3, YEF3A, YLR249W
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand SO4 13 x SO4
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 98.8Å b: 107.7Å c: 209.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.242
Expression system: Saccharomyces cerevisiae

Quick links

Citations

25 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
24 more

7 mentions without citation

Insights into the domain and repeat architecture of target of rapamycin.
Knutson BA. (2010)
6 more