› 2iwc

X-ray diffraction
2.1Å resolution

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methicillin resistance mecR1 protein Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 30.23 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A0B0 (Residues: 333-585; Coverage: 43%)
Gene names: SA0039, mecR, mecR1
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand PNM 1 x PNM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P41212
Unit cell:
a: 58.4Å b: 58.4Å c: 148.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Cell envelope stress response in Gram-positive bacteria.
Jordan et al. (2008)
4 more

2 mentions without citation

An experiment-informed signal transduction model for the role of the Staphylococcus aureus MecR1 protein in β-lactam resistance.
Belluzo et al. (2019)
1 more