› 2iwj

Solution NMR

SOLUTION STRUCTURE OF THE ZN COMPLEX OF HIV-2 NCP(23-49) PEPTIDE, ENCOMPASSING PROTEIN CCHC-LINKER, DISTAL CCHC ZN-BINDING MOTIF AND C- TERMINAL TAIL.

Released:
DOI: 10.2210/pdb2iwj/pdb
PDB entry 2iwj coloured by chain, ensemble of 30 models, front view.
PDB entry 2iwj coloured by chain, ensemble of 30 models, side view.
PDB entry 2iwj coloured by chain, ensemble of 30 models, top view.
Source organism: Human immunodeficiency virus 2
Entry authors: Amodeo P, Castiglione Morelli MA, Ostuni A, Cristinziano P, Bavoso A

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Endopeptidase for which the P1 residue is preferably hydrophobic.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nucleocapsid protein p7 Chain: A
Molecule details ›
Chain: A
Length: 27 amino acids
Theoretical weight: 3.02 KDa
Source organism: Human immunodeficiency virus 2
Expression system: Not provided
UniProt:
  • Canonical: P18042 (Residues: 406-432; Coverage: 2%)
Gene name: gag-pol
Sequence domains: Zinc knuckle

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 50%
Refinement method: SIMULATED ANNEALING-ENERGY MINIMIZATION
Expression system: Not provided

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