› 2ix1

X-ray diffraction
2.74Å resolution

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151827 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Exoribonuclease 2 Chain: A
Molecule details ›
Chain: A
Length: 664 amino acids
Theoretical weight: 74.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30850 (Residues: 1-644; Coverage: 100%)
Gene names: JW1279, b1286, rnb
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP *AP*AP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 4.23 KDa
Source organism: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P65
Unit cell:
a: 86.316Å b: 86.316Å c: 279.245Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

30 review citations

The critical role of RNA processing and degradation in the control of gene expression.
Arraiano et al. (2010)
29 more

23 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
22 more