› 2ix4

X-ray diffraction
1.95Å resolution

Arabidopsis thaliana mitochondrial beta-ketoacyl ACP synthase hexanoic acid complex

Released:
DOI: 10.2210/pdb2ix4/pdb
PDB entry 2ix4 coloured by chain, front view.
PDB entry 2ix4 coloured by chain, side view.
PDB entry 2ix4 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structure of the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase.
Christensen CE, Kragelund BB, von Wettstein-Knowles P, Henriksen A
Protein Sci 16 261-72 (2007)
PMID: 17242430

Function and Biology Details

Reaction catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 45.96 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8L3X9 (Residues: 31-461; Coverage: 94%)
Gene names: At2g04540, KAS, T1O3.5
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand 6NA 2 x 6NA
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 72.109Å b: 92.942Å c: 74.217Å
α: 90° β: 106.59° γ: 90°
R-values:
R R work R free
0.197 0.195 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Current understanding of fatty acid biosynthesis and the acyl carrier protein.
Chan et al. (2010)
4 more

4 mentions without citation

Dimeric interactions and complex formation using direct coevolutionary couplings.
dos Santos et al. (2015)
3 more