› 2ixg

X-ray diffraction
2.7Å resolution

Crystal structure of the ATPase domain of TAP1 with ATP (S621A, G622V, D645N mutant)

Released:
DOI: 10.2210/pdb2ixg/pdb
PDB entry 2ixg coloured by chain, front view.
PDB entry 2ixg coloured by chain, side view.
PDB entry 2ixg coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Distinct structural and functional properties of the ATPase sites in an asymmetric ABC transporter.
Procko E, Ferrin-O'Connell I, Ng SL, Gaudet R
Mol Cell 24 51-62 (2006)
PMID: 17018292

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + antigen peptide(Side 1) = ADP + phosphate + antigen peptide(Side 2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antigen peptide transporter 1 Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 29.51 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36370 (Residues: 465-725; Coverage: 36%)
Gene names: Abcb2, Mtp1, Tap1
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P41212
Unit cell:
a: 67.477Å b: 67.477Å c: 105.903Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.212 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

23 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
22 more