› 2ixs

X-ray diffraction
2Å resolution

Structure of SdaI restriction endonuclease

Released:
DOI: 10.2210/pdb2ixs/pdb
PDB entry 2ixs coloured by chain, front view.
PDB entry 2ixs coloured by chain, side view.
PDB entry 2ixs coloured by chain, top view.
Source organism: Streptomyces diastaticus
Primary publication:
The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture.
Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V
Structure 14 1389-400 (2006)
PMID: 16962970

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
R.SdaI Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.53 KDa
Source organism: Streptomyces diastaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: C4P954 (Residues: 1-323; Coverage: 100%)
Gene name: sdaIR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 9 x SO4
Ligand EPE 2 x EPE
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2221
Unit cell:
a: 66.901Å b: 237.991Å c: 111.404Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.178 0.212
Expression system: Escherichia coli

Quick links

Citations

18 citation in other articles

Structural and evolutionary classification of Type II restriction enzymes based on theoretical and experimental analyses.
Orlowski et al. (2008)
17 more

3 mentions without citation

The N-terminal domain of the arenavirus L protein is an RNA endonuclease essential in mRNA transcription.
Morin et al. (2010)
2 more