› 2iy3

Electron Microscopy
16Å resolution

Structure of the E. Coli Signal Regognition Particle

Released:
DOI: 10.2210/pdb2iy3/pdb
PDB entry 2iy3 coloured by chain, front view.
PDB entry 2iy3 coloured by chain, side view.
PDB entry 2iy3 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the E. coli signal recognition particle bound to a translating ribosome.
Schaffitzel C, Oswald M, Berger I, Ishikawa T, Abrahams JP, Koerten HK, Koning RI, Ban N
Nature 444 503-6 (2006)
PMID: 17086205
Related structures: EMD-1250

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126574 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Signal recognition particle 54 kDa protein; Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 432 amino acids
Theoretical weight: 48.27 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O07347 (Residues: 1-296; Coverage: 69%)
  • Canonical: Q97ZE7 (Residues: 297-432; Coverage: 30%)
Gene names: SSO0971, ffh, srp54
Sequence domains:
SIGNAL SEQUENCE Chain: C
Molecule details ›
Chain: C
Length: 17 amino acids
Theoretical weight: 1.38 KDa
Source organism: synthetic construct
Expression system: Not provided
4.5S RNA Chain: B
Molecule details ›
Chain: B
Length: 110 nucleotides
Theoretical weight: 35.55 KDa
Sequence domains: Bacterial small signal recognition particle RNA

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 16Å
Relevant EMDB volumes: EMD-1250
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

27 review citations

Protein translocation across the bacterial cytoplasmic membrane.
Driessen et al. (2008)
26 more

3 mentions without citation

Robots, pipelines, polyproteins: enabling multiprotein expression in prokaryotic and eukaryotic cells.
Vijayachandran et al. (2011)
2 more