› 2iy6

X-ray diffraction
1.8Å resolution

1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE FROM THERMUS WITH BOUND CITRATE

Released:
DOI: 10.2210/pdb2iy6/pdb
PDB entry 2iy6 coloured by chain, front view.
PDB entry 2iy6 coloured by chain, side view.
PDB entry 2iy6 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of Thermus thermophilus Delta1-pyrroline-5-carboxylate dehydrogenase.
Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov TH
J Mol Biol 362 490-501 (2006)
PMID: 16934832

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamate gamma-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 516 amino acids
Theoretical weight: 57.11 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SI02 (Residues: 1-516; Coverage: 100%)
Gene name: TTHA1578
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand FLC 2 x FLC
Ligand MPD 8 x MPD
Ligand MRD 5 x MRD
Ligand NA 2 x NA
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: R3
Unit cell:
a: 102.047Å b: 102.047Å c: 278.901Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.14 0.139 0.17
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Structural biology of proline catabolism.
Tanner JJ. (2008)
6 more