› 2iyk

X-ray diffraction
2.95Å resolution

Crystal structure of the UPF2-interacting domain of nonsense mediated mRNA decay factor UPF1

Released:
DOI: 10.2210/pdb2iyk/pdb
PDB entry 2iyk coloured by chain, front view.
PDB entry 2iyk coloured by chain, side view.
PDB entry 2iyk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the UPF2-interacting domain of nonsense-mediated mRNA decay factor UPF1.
Kadlec J, Guilligay D, Ravelli RB, Cusack S
RNA 12 1817-24 (2006)
PMID: 16931876

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulator of nonsense transcripts 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q92900 (Residues: 115-272; Coverage: 14%)
Gene names: KIAA0221, RENT1, UPF1
Sequence domains: RNA helicase (UPF2 interacting domain)

Ligands and Environments

1 bound ligand:
Ligand ZN 6 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 64.56Å b: 72.98Å c: 73.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Nonsense-mediated mRNA decay: an intricate machinery that shapes transcriptomes.
Lykke-Andersen et al. (2015)
20 more

7 mentions without citation

No-nonsense: insights into the functional interplay of nonsense-mediated mRNA decay factors.
Mailliot et al. (2022)
6 more