› 2izo

X-ray diffraction
2.9Å resolution

Structure of an Archaeal PCNA1-PCNA2-FEN1 Complex

Released:
DOI: 10.2210/pdb2izo/pdb
PDB entry 2izo coloured by chain, front view.
PDB entry 2izo coloured by chain, side view.
PDB entry 2izo coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Structure of an archaeal PCNA1-PCNA2-FEN1 complex: elucidating PCNA subunit and client enzyme specificity.
Doré AS, Kilkenny ML, Jones SA, Oliver AW, Roe SM, Bell SD, Pearl LH
Nucleic Acids Res 34 4515-26 (2006)
PMID: 16945955

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-157632 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Flap endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 346 amino acids
Theoretical weight: 39.36 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980U8 (Residues: 5-349; Coverage: 99%)
Gene names: SSO0179, fen
Sequence domains:
DNA polymerase sliding clamp 2 Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 27.55 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z84 (Residues: 1-245; Coverage: 100%)
Gene names: SSO1047, pcn2, pcnA-2
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 1 Chain: C
Molecule details ›
Chain: C
Length: 249 amino acids
Theoretical weight: 27.57 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P57766 (Residues: 1-249; Coverage: 100%)
Gene names: C41_008, SSO0397, pcn1, pcnA-like
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 93.986Å b: 99.774Å c: 99.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.25 0.312
Expression system: Escherichia coli

Quick links

Citations

11 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
10 more

5 mentions without citation

Coordination of multiple enzyme activities by a single PCNA in archaeal Okazaki fragment maturation.
Beattie et al. (2012)
4 more