› 2j0k

X-ray diffraction
3Å resolution

Crystal structure of a fragment of focal adhesion kinase containing the FERM and kinase domains.

Released:
DOI: 10.2210/pdb2j0k/pdb
PDB entry 2j0k coloured by chain, front view.
PDB entry 2j0k coloured by chain, side view.
PDB entry 2j0k coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structural basis for the autoinhibition of focal adhesion kinase.
Lietha D, Cai X, Ceccarelli DF, Li Y, Schaller MD, Eck MJ
Cell 129 1177-87 (2007)
PMID: 17574028

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Focal adhesion kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 656 amino acids
Theoretical weight: 75.32 KDa
Source organism: Gallus gallus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q00944 (Residues: 31-686; Coverage: 62%)
Gene names: FAK, FAK1, PTK2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 4ST 2 x 4ST
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 63.022Å b: 106.209Å c: 240.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.299
Expression system: Trichoplusia ni

Quick links

Citations

81 review citations

Cell adhesion: integrating cytoskeletal dynamics and cellular tension.
Parsons et al. (2010)
80 more

4 mentions without citation

FAK dimerization controls its kinase-dependent functions at focal adhesions.
Brami-Cherrier et al. (2014)
3 more