› 2j0n

X-ray diffraction
2.1Å resolution

A proteolytically truncated form of Shigella Flexneri IpaD

Released:
DOI: 10.2210/pdb2j0n/pdb
PDB entry 2j0n coloured by chain, front view.
PDB entry 2j0n coloured by chain, side view.
PDB entry 2j0n coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 301
Primary publication:
Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.
Johnson S, Roversi P, Espina M, Olive A, Deane JE, Birket S, Field T, Picking WD, Blocker AJ, Galyov EE, Picking WL, Lea SM
J Biol Chem 282 4035-44 (2007)
PMID: 17077085

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148256 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Invasin IpaD Chains: A, B
Molecule details ›
Chains: A, B
Length: 200 amino acids
Theoretical weight: 22.15 KDa
Source organism: Shigella flexneri 2a str. 301
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18013 (Residues: 133-332; Coverage: 60%)
Gene names: CP0126, ipaD
Sequence domains: Type III secretion systems tip complex components
Structure domains: IpaD-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 77.79Å b: 91.36Å c: 54.91Å
α: 90° β: 96.35° γ: 90°
R-values:
R R work R free
0.19 0.189 not available
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

34 review citations

Molecular pathogenesis of Shigella spp.: controlling host cell signaling, invasion, and death by type III secretion.
Schroeder et al. (2008)
33 more

5 mentions without citation

Self-chaperoning of the type III secretion system needle tip proteins IpaD and BipD.
Johnson et al. (2007)
4 more