› 2j2f

X-ray diffraction
2.65Å resolution

The T199D Mutant of Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean)

Released:
DOI: 10.2210/pdb2j2f/pdb
PDB entry 2j2f coloured by chain, front view.
PDB entry 2j2f coloured by chain, side view.
PDB entry 2j2f coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
A single mutation in the castor Delta9-18:0-desaturase changes reaction partitioning from desaturation to oxidase chemistry.
Guy JE, Abreu IA, Moche M, Lindqvist Y, Whittle E, Shanklin J
Proc Natl Acad Sci U S A 103 17220-4 (2006)
PMID: 17088542

Function and Biology Details

Reaction catalysed: 
Stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 363 amino acids
Theoretical weight: 41.72 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22337 (Residues: 34-396; Coverage: 92%)
Sequence domains: Fatty acid desaturase
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

1 bound ligand:
Ligand FE 12 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 82.049Å b: 145.768Å c: 193.245Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.272
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Biosynthesis and function of polyacetylenes and allied natural products.
Minto et al. (2008)
3 more

1 mention without citation

A single mutation in the castor Delta9-18:0-desaturase changes reaction partitioning from desaturation to oxidase chemistry.
Guy et al. (2006)