› 2j3w

X-ray diffraction
2.1Å resolution

The crystal structure of the bet3-trs31-sedlin complex.

Released:
DOI: 10.2210/pdb2j3w/pdb
PDB entry 2j3w coloured by chain, front view.
PDB entry 2j3w coloured by chain, side view.
PDB entry 2j3w coloured by chain, top view.
Source organisms:
Primary publication:
The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering.
Kim YG, Raunser S, Munger C, Wagner J, Song YL, Cygler M, Walz T, Oh BH, Sacher M
Cell 127 817-30 (2006)
PMID: 17110339

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Trafficking protein particle complex subunit 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CQP2 (Residues: 1-140; Coverage: 100%)
Gene names: Sedl, Trappc2
Sequence domains: Sedlin, N-terminal conserved region
Structure domains: Beta-Lactamase
Trafficking protein particle complex subunit 5 Chains: B, F
Molecule details ›
Chains: B, F
Length: 188 amino acids
Theoretical weight: 21.06 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DGL5 (Residues: 1-188; Coverage: 100%)
Gene names: trappc5, wu:fc34a11, zgc:92866
Sequence domains: Transport protein particle (TRAPP) component
Structure domains: Trafficking protein particle complex subunit 3
Trafficking protein particle complex subunit 3 Chains: D, E
Molecule details ›
Chains: D, E
Length: 182 amino acids
Theoretical weight: 20.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O55013 (Residues: 1-180; Coverage: 100%)
Gene names: Bet3, Trappc3
Sequence domains: Transport protein particle (TRAPP) component
Structure domains: Trafficking protein particle complex subunit 3

Ligands and Environments

1 bound ligand:
Ligand PLM 2 x PLM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: C2
Unit cell:
a: 232.211Å b: 63.063Å c: 72.018Å
α: 90° β: 91.72° γ: 90°
R-values:
R R work R free
0.23 0.23 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

40 review citations

Role of Rab GTPases in membrane traffic and cell physiology.
Hutagalung et al. (2011)
39 more

8 mentions without citation

Discovery of new Longin and Roadblock domains that form platforms for small GTPases in Ragulator and TRAPP-II.
Levine et al. (2013)
7 more