› 2j40

X-ray diffraction
2.1Å resolution

1-pyrroline-5-carboxylate dehydrogenase from Thermus thermophilus with bound inhibitor L-proline and NAD.

Released:
DOI: 10.2210/pdb2j40/pdb
PDB entry 2j40 coloured by chain, front view.
PDB entry 2j40 coloured by chain, side view.
PDB entry 2j40 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Inagaki E, Sakamoto K, Nishio M, Yokoyama S

Function and Biology Details

Reaction catalysed: 
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177924 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamate gamma-semialdehyde dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 516 amino acids
Theoretical weight: 57.13 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SI02 (Residues: 1-516; Coverage: 100%)
Gene name: TTHA1578
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
5 bound ligands:
Ligand PRO 2 x PRO
Ligand ACT 4 x ACT
Ligand MRD 2 x MRD
Ligand NA 2 x NA
Ligand MPD 4 x MPD
1 modified residue:
Ligand CSO 2 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: R3
Unit cell:
a: 102.043Å b: 102.043Å c: 278.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.142 0.14 0.189
Expression system: Escherichia coli BL21(DE3)

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