› 2j41

X-ray diffraction
1.9Å resolution

Crystal structure of Staphylococcus aureus guanylate monophosphate kinase

Released:
DOI: 10.2210/pdb2j41/pdb
PDB entry 2j41 coloured by chain, front view.
PDB entry 2j41 coloured by chain, side view.
PDB entry 2j41 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure of Staphylococcus aureus guanylate monophosphate kinase.
El Omari K, Dhaliwal B, Lockyer M, Charles I, Hawkins AR, Stammers DK
Acta Crystallogr Sect F Struct Biol Cryst Commun 62 949-53 (2006)
PMID: 17012781

Function and Biology Details

Reaction catalysed: 
ATP + GMP = ADP + GDP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 207 amino acids
Theoretical weight: 24.07 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5HGM3 (Residues: 1-207; Coverage: 100%)
Gene names: SACOL1221, gmk
Sequence domains: Guanylate kinase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 5 x SO4
Ligand K 3 x K
Ligand 5GP 3 x 5GP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 70.012Å b: 93.986Å c: 83.936Å
α: 90° β: 110.59° γ: 90°
R-values:
R R work R free
0.209 0.207 0.239
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Molecular mechanism and evolution of guanylate kinase regulation by (p)ppGpp.
Liu et al. (2015)
9 more

1 mention without citation

Structure of Staphylococcus aureus guanylate monophosphate kinase.
El Omari et al. (2006)