› 2j4f

X-ray diffraction
2.8Å resolution

Torpedo acetylcholinesterase - Hg heavy-atom derivative

Released:
DOI: 10.2210/pdb2j4f/pdb
PDB entry 2j4f coloured by chain, front view.
PDB entry 2j4f coloured by chain, side view.
PDB entry 2j4f coloured by chain, top view.
Source organism: Tetronarce californica
Primary publication:
A metastable state of Torpedo californica acetylcholinesterase generated by modification with organomercurials.
Kreimer DI, Dolginova EA, Raves M, Sussman JL, Silman I, Weiner L
Biochemistry 33 14407-18 (1994)
PMID: 7981200

Function and Biology Details

Reaction catalysed: 
Acetylcholine + H(2)O = choline + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137375 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholinesterase Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 61.33 KDa
Source organism: Tetronarce californica
UniProt:
  • Canonical: P04058 (Residues: 22-564; Coverage: 96%)
Gene name: ache
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand HG 2 x HG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 110.7Å b: 110.7Å c: 135Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.262

Quick links

Citations

14 citation in other articles

Mechanisms of cholinesterase inhibition by inorganic mercury.
Frasco et al. (2007)
13 more

2 mentions without citation

Nuclear AURKA acquires kinase-independent transactivating function to enhance breast cancer stem cell phenotype.
Zheng et al. (2016)
1 more