› 2j5d

Solution NMR

NMR structure of BNIP3 transmembrane domain in lipid bicelles

Released:
DOI: 10.2210/pdb2j5d/pdb
PDB entry 2j5d coloured by chain, ensemble of 16 models, front view.
PDB entry 2j5d coloured by chain, ensemble of 16 models, side view.
PDB entry 2j5d coloured by chain, ensemble of 16 models, top view.
Source organism: Homo sapiens
Primary publication:
Unique dimeric structure of BNip3 transmembrane domain suggests membrane permeabilization as a cell death trigger.
Bocharov EV, Pustovalova YE, Pavlov KV, Volynsky PE, Goncharuk MV, Ermolyuk YS, Karpunin DV, Schulga AA, Kirpichnikov MP, Efremov RG, Maslennikov IV, Arseniev AS
J Biol Chem 282 16256-66 (2007)
PMID: 17412696

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 45 amino acids
Theoretical weight: 4.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12983 (Residues: 146-190; Coverage: 23%)
Gene names: BNIP3, NIP3
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 74%
Refinement method: TAD
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Role of BNIP3 and NIX in cell death, autophagy, and mitophagy.
Zhang et al. (2009)
16 more

9 mentions without citation

Single-spanning transmembrane domains in cell growth and cell-cell interactions: More than meets the eye?
Hubert et al. (2010)
8 more