Function and Biology Details
Reaction catalysed:
(1a) feruloyl-CoA + H(2)O = 3-hydroxy-3-(4-hydroxy-3-methoxyphenyl)propanoyl-CoA
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero hexamer (preferred)
Assembly name:
Hydroxycinnamoyl-CoA hydratase-lyase (preferred)
PDBe Complex ID:
PDB-CPX-130664 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Hydroxycinnamoyl-CoA hydratase-lyase
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.01 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains:
Length: 276 amino acids
Theoretical weight: 31.01 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
O69762 (Residues: 1-276; Coverage: 100%)
Structure domains:
Hydroxycinnamoyl-CoA hydratase-lyase
Molecule details ›
Chains: B, C, D, E, F, G, H, J, K, L
Length: 276 amino acids
Theoretical weight: 31.04 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains:
Length: 276 amino acids
Theoretical weight: 31.04 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O69762 (Residues: 1-276; Coverage: 100%)
Structure domains:
Hydroxycinnamoyl-CoA hydratase-lyase
Molecule details ›
Chain: I
Length: 276 amino acids
Theoretical weight: 31.04 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains:
Length: 276 amino acids
Theoretical weight: 31.04 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: O69762 (Residues: 1-276; Coverage: 100%)
Structure domains:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-1
Spacegroup:
P21212
Expression system: Escherichia coli BL21(DE3)
