› 2j5x

X-ray diffraction
2.8Å resolution

STRUCTURE OF THE SMALL G PROTEIN ARF6 IN COMPLEX WITH GTPGAMMAS

Released:
DOI: 10.2210/pdb2j5x/pdb
PDB entry 2j5x coloured by chain, front view.
PDB entry 2j5x coloured by chain, side view.
PDB entry 2j5x coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The structural GDP/GTP cycle of human Arf6.
Pasqualato S, Ménétrey J, Franco M, Cherfils J
EMBO Rep 2 234-8 (2001)
PMID: 11266366

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-ribosylation factor 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 175 amino acids
Theoretical weight: 20.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62330 (Residues: 1-175; Coverage: 100%)
Gene name: ARF6
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand GSP 2 x GSP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P41212
Unit cell:
a: 72.895Å b: 72.895Å c: 131.489Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.276
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Mechanisms of endocytosis.
Doherty et al. (2009)
18 more

21 mentions without citation

Predicting data quality in biological X-ray solution scattering.
Wang et al. (2018)
20 more