› 2j63

X-ray diffraction
2.48Å resolution

Crystal structure of AP endonuclease LMAP from Leishmania major

Released:
DOI: 10.2210/pdb2j63/pdb
PDB entry 2j63 coloured by chain, front view.
PDB entry 2j63 coloured by chain, side view.
PDB entry 2j63 coloured by chain, top view.
Source organism: Leishmania major
Primary publication:
Crystal structure and DNA repair activities of the AP endonuclease from Leishmania major.
Vidal AE, Harkiolaki M, Gallego C, Castillo-Acosta VM, Ruiz-Pérez LM, Wilson K, González-Pacanowska D
J Mol Biol 373 827-38 (2007)
PMID: 17870086

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA-(apurinic or apyrimidinic site) endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 467 amino acids
Theoretical weight: 50.87 KDa
Source organism: Leishmania major
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15922 (Residues: 1-447; Coverage: 100%)
Gene names: AP-endonuclease, LMJF_16_0680, LmAP
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 167.103Å b: 45.009Å c: 115.134Å
α: 90° β: 116.79° γ: 90°
R-values:
R R work R free
0.203 0.2 0.246
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

DNA repair pathways in trypanosomatids: from DNA repair to drug resistance.
Genois et al. (2014)
2 more