› 2j6a

X-ray diffraction
1.7Å resolution

Structure of S. cerevisiae Trm112 protein, a methyltransferase activator

Released:
DOI: 10.2210/pdb2j6a/pdb
PDB entry 2j6a coloured by chain, front view.
PDB entry 2j6a coloured by chain, side view.
PDB entry 2j6a coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The zinc finger protein Ynr046w is plurifunctional and a component of the eRF1 methyltransferase in yeast.
Heurgué-Hamard V, Graille M, Scrima N, Ulryck N, Champ S, van Tilbeurgh H, Buckingham RH
J Biol Chem 281 36140-8 (2006)
PMID: 17008308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multifunctional methyltransferase subunit TRM112 Chain: A
Molecule details ›
Chain: A
Length: 141 amino acids
Theoretical weight: 15.91 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53738 (Residues: 1-135; Coverage: 100%)
Gene names: N3445, TRM112, YNR046W
Sequence domains: Trm112p-like protein
Structure domains: N-terminal domain of TfIIb

Ligands and Environments

2 bound ligands:
Ligand EDO 1 x EDO
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 92.6Å b: 38.46Å c: 45.81Å
α: 90° β: 102.24° γ: 90°
R-values:
R R work R free
0.2 0.198 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Methylation of proteins involved in translation.
Polevoda et al. (2007)
5 more

6 mentions without citation

Prediction of structures of zinc-binding proteins through explicit modeling of metal coordination geometry.
Wang et al. (2010)
5 more