› 2j6h

X-ray diffraction
2.35Å resolution

E. coli glucosamine-6-P synthase in complex with glucose-6P and 5-oxo- L-norleucine

Released:
DOI: 10.2210/pdb2j6h/pdb
PDB entry 2j6h coloured by chain, front view.
PDB entry 2j6h coloured by chain, side view.
PDB entry 2j6h coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase.
Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B
J Biol Chem 281 4404-12 (2006)
PMID: 16339762

Function and Biology Details

Reaction catalysed: 
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147884 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] Chains: A, B
Molecule details ›
Chains: A, B
Length: 608 amino acids
Theoretical weight: 66.85 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P17169 (Residues: 2-609; Coverage: 100%)
Gene names: JW3707, b3729, glmS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ONL 2 x ONL
Ligand G6Q 2 x G6Q
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 83.3Å b: 91.23Å c: 184.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.237
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Gates of enzymes.
Gora et al. (2013)
9 more

8 mentions without citation

The gates of ion channels and enzymes.
Zhou et al. (2010)
7 more