› 2j6y

X-ray diffraction
1.85Å resolution

Structural and Functional Characterisation of partner switching regulating the environmental stress response in Bacillus subtilis

Released:
DOI: 10.2210/pdb2j6y/pdb
PDB entry 2j6y coloured by chain, front view.
PDB entry 2j6y coloured by chain, side view.
PDB entry 2j6y coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structural and functional characterization of partner switching regulating the environmental stress response in Bacillus subtilis.
Hardwick SW, Pané-Farré J, Delumeau O, Marles-Wright J, Murray JW, Hecker M, Lewis RJ
J Biol Chem 282 11562-72 (2007)
PMID: 17303566

Function and Biology Details

Reaction catalysed: 
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoserine phosphatase RsbU Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 111 amino acids
Theoretical weight: 13.18 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40399 (Residues: 1-111; Coverage: 33%)
Gene names: BSU04700, rsbU
Sequence domains: Phosphoserine phosphatase RsbU, N-terminal domain
Structure domains: KaiA/RbsU domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 100.157Å b: 47.831Å c: 94.968Å
α: 90° β: 104.36° γ: 90°
R-values:
R R work R free
0.186 0.183 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Stress responses of bacteria.
Marles-Wright et al. (2007)
3 more