› 2j74

X-ray diffraction
2.6Å resolution

Structure of Beta-1,4-Galactanase

Released:
DOI: 10.2210/pdb2j74/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 2j74 coloured by chain, front view.
PDB entry 2j74 coloured by chain, side view.
PDB entry 2j74 coloured by chain, top view.
Source organism: Bacillus licheniformis
Primary publication:
Investigating the binding of beta-1,4-galactan to Bacillus licheniformis beta-1,4-galactanase by crystallography and computational modeling.
Le Nours J, De Maria L, Welner D, Jørgensen CT, Christensen LL, Borchert TV, Larsen S, Lo Leggio L
Proteins 75 977-89 (2009)
PMID: 19089956

Function and Biology Details

Reaction catalysed: 
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179196 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Endo-beta-1,4-galactanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 399 amino acids
Theoretical weight: 43.78 KDa
Source organism: Bacillus licheniformis
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q65CX5 (Residues: 26-424; Coverage: 100%)
Gene names: BL00263, BLi04276, galA, ganB, yvfO
Sequence domains: Glycosyl hydrolase family 53
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL
Carbohydrate polymer
1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21
Unit cell:
a: 50.43Å b: 80.25Å c: 104.5Å
α: 90° β: 99.5° γ: 90°
R-values:
R R work R free
0.202 0.202 0.239
Expression system: Bacillus subtilis

Quick links

Citations

1 review citation

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
6 other articles

3 mentions without citation

Structure of Aspergillus aculeatus β-1,4-galactanase in complex with galactobiose.
Torpenholt et al. (2019)
2 more