› 2j7n

X-ray diffraction
2.3Å resolution

Structure of the RNAi polymerase from Neurospora crassa

Released:
DOI: 10.2210/pdb2j7n/pdb
PDB entry 2j7n coloured by chain, front view.
PDB entry 2j7n coloured by chain, side view.
PDB entry 2j7n coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
The structure of an RNAi polymerase links RNA silencing and transcription.
Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM
PLoS Biol 4 e434 (2006)
PMID: 17147473

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195407 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-dependent RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1022 amino acids
Theoretical weight: 116.71 KDa
Source organism: Neurospora crassa
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9Y7G6 (Residues: 381-1402; Coverage: 73%)
Gene name: qde-1
Sequence domains: RNA dependent RNA polymerase
Structure domains: RNA-dependent RNA polymerase, slab domain, helical subdomain-like

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 101.021Å b: 122.553Å c: 114.701Å
α: 90° β: 108.9° γ: 90°
R-values:
R R work R free
0.219 0.217 0.264
Expression system: Saccharomyces cerevisiae

Quick links

Citations

21 review citations

Origins and evolution of eukaryotic RNA interference.
Shabalina et al. (2008)
20 more

11 mentions without citation

Non-canonical DNA transcription enzymes and the conservation of two-barrel RNA polymerases.
Ruprich-Robert et al. (2010)
10 more